1. Impact of maternal aging on the molecular signature of human cumulus cells 
Susanna McReynolds, Ph.D., Monika Dzieciatkowska, Ph.D., Blair R. McCallie, B.S., Shane D. Mitchell, B.S., John Stevens, B.S., Kirk Hansen, Ph.D., William B. Schoolcraft, M.D., Mandy G. Katz-Jaffe, Ph.D. 
Fertility and Sterility 
Volume 98, Issue 6, Pages e5 (December 2012)
DOI: /j.fertnstert
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Q RT PCR of OP genes. The expression of ATP5I and NDUFA1 relative to the housekeeping gene PPIA revealed significant decreased expression in AMA infertile women compared with controls in concordance with MS/MS data (*P<.05). The expression of ATP6V1A and COX10 relative to the housekeeping gene PPIA revealed significant increased expression in AMA infertile women compared with controls (*P<.05).
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Lipid metabolism was observed to be altered in CCs relative to maternal aging. (A) Representative western blot of the protein ACAT2 relative to the housekeeping protein Beta-Tubulin showing a 1.5-fold increased expression in CCs from AMA infertile women compared with controls. (B) Q RT PCR of lipid metabolism genes. The expression of ACSL3, CPT2, FASN, SCD, TP53, ACAT2, HSD17B4-1, and HSD17B4-2 relative to the housekeeping gene PPIA revealed significant increased expression in AMA infertile women compared with controls (*P<.05).
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
The pre-mRNA splicing pathway was observed to be altered in CCs relative to maternal aging. (A) Representative western blot of the proteins SFPQ, KHSRP, and DDX46 relative to the housekeeping protein Beta-Tubulin. SFPQ shows a 2.1-fold decrease in expression, KHSRP a 2.8-fold decrease, and DDX46 a 2.6-fold decrease in protein expression in CCs from AMA infertile women compared with controls. (B) QRT PCR of pre-mRNA splicing genes. The expression of SFPQ and SNRPF relative to the housekeeping gene PPIA revealed significant decreased expression in AMA infertile women compared with controls in concordance with the proteomic data (*P<.05).
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 1 Flow chart of experimental design.
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6. Supplemental Figure 2
Functional annotation of differentially expressed proteins. Pie charts display predominant GO biological processes of (A) proteins with increased expression and (B) proteins with decreased expression in relation to AMA.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions