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Endoluminal smooth muscle cell seeding limits intimal hyperplasia

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Presentation on theme: "Endoluminal smooth muscle cell seeding limits intimal hyperplasia"— Presentation transcript:

1 Endoluminal smooth muscle cell seeding limits intimal hyperplasia
Dominique Gomes, MD, Liliane Louedec, Didier Plissonnier, MD, Marie-Christine Dauge, MD, Dominique Henin, MD, Mary Osborne- Pellegrin, PhD, Jean-Baptiste Michel, MD  Journal of Vascular Surgery  Volume 34, Issue 4, Pages (October 2001) DOI: /mva Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

2 Fig. 1 En face preparations of aorta showing luminal surface for evaluation of deendothelialization. A, Limit between deendothelialized area (a ) and intact area (b ) (×10). B, Nuclei of endothelial cells (white arrow ) and of SMCs (black arrow ) present different shapes and orientations (×40). Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

3 Fig. 2 Transverse (A) and longitudinal (B) sections of deendothelialized aorta, seeded with SMCs labeled with fluorescent DilC18 at 2 days (×10). Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

4 Fig. 3 Histograms representing nuclear content, collagen content, and elastin content, in intima of deendothelialized and allografted aortas. DE, Deendothelialized aorta; DE-SMC, deendothelialization followed by SMC seeding; DE-FIB, deendothelialization followed by FIB seeding; ALLO, control allograft; ALLO-DE, deendothelialized allograft; ALLO-SMC, deendothelialized allografts followed by SMC seeding; ALLO-FIB, deendothelialized allografts followed by FIB seeding. *P <.05; ***P <.001. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

5 Fig. 4 Histologic sections of paraffin-embedded deendothelialized aortas at 2 months, with selective staining: nuclei were stained with Harris' hematoxylin, elastin was stained with orcein, and collagen was stained with Sirius red (×40). DE, Deendothelialized aorta; DE-SMC, deendothelialization followed by SMC seeding; DE-FIB, deendothelialization followed by FIB seeding. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

6 Fig. 5 Histologic sections of paraffin-embedded allografts at 2 months, with selective staining: nuclei were stained with Harris' hematoxylin, elastin was stained with orcein, and collagen was stained with Sirius red (×40). ALLO-DE, Deendothelialized allograft; ALLO-SMC, deendothelialized allograft followed by SMC seeding; ALLO-FIB, deendothelialized allograft followed by FIB seeding. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

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