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Figure 3. Verification of GluRd2 as the target antigen of the patient's antineural autoantibodies Verification of GluRd2 as the target antigen of the patient's.

Published byDana Havlíčková Modified over 5 years ago

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Presentation on theme: "Figure 3. Verification of GluRd2 as the target antigen of the patient's antineural autoantibodies Verification of GluRd2 as the target antigen of the patient's."— Presentation transcript:

1 Figure 3. Verification of GluRd2 as the target antigen of the patient's antineural autoantibodies
Verification of GluRd2 as the target antigen of the patient's antineural autoantibodies (A) Immunofluorescence analysis of transfected HEK293 cells. The patient's serum was incubated diluted 1:100 on formalin-fixed recombinant HEK293 cells expressing GluRd2 (A.a) or a mock-transfected control with empty vector (A.b). (B) Neutralization of immunofluorescence reaction on cerebellum (rat: B.a, B.b; monkey: B.c, B.d). The patient's serum diluted 1:320 was preincubated with extracts of HEK293 cells transfected with the GluRd2 (B.a, B.c) or with empty vector as control (B.b, B.d). The extract containing the GluRd2 abolished the immune reaction. Scale bar: 50 μm. (C–E) Immunofluorescence staining of recombinant HEK293 expressing GluRd2 (C.a–C.c), rat cerebellum (D.a–D.c), and monkey cerebellum (E.a–E.c). HEK293 and tissue sections with patient's serum diluted 1:100 (green, C.a–E.a) and 1:500 polyclonal rabbit anti-GluRd2 antibody (red, C.b–E.b). The merged images display colocalization of both reactivities (C.c–E.c). Scale bar: 50 μm. Ramona Miske et al. Neurol Neuroimmunol Neuroinflamm 2016;3:e255 © 2016 American Academy of Neurology

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