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Marita Pall, M. D. , Ph. D. , Margaret Nguyen, B. S

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1 Effect of sex steroids and insulin on dehydroepiandrosterone sulfate production by hepatoma G2 cells 
Marita Pall, M.D., Ph.D., Margaret Nguyen, B.S., Denis Magoffin, Ph.D., Ricardo Azziz, M.D., M.P.H., M.B.A.  Fertility and Sterility  Volume 91, Issue 6, Pages (June 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Cumulative DHEAS production after addition of DHEA substrate to HepG2 cell cultures. As compared with 12 hours, ∗P=.0006, ∗∗P=.07, and ∗∗∗P=.02. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Dehydroepiandrosterone sulfate in HepG2 cell culture media after incubation for 12 to 72 hours. ∗P<.05 as compared with 12 and 24 hours. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Cumulative DHEAS production after incubation of HepG2 cells with DHEA only (10−5 mol/L), coincubation of DHEA (10−5 mol/L) with T (10−6 mol/L), DHEA (10−5 mol/L) with DHT (10−6 mol/L), and DHEA (10−5 mol/L) with E2 (10−6 mol/L). As compared with DHEA only at 24 hours, ∗P=.01 and ∗∗P=.08. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

5 Figure 4 Cumulative DHEAS production after incubation of HepG2 cells with DHEA only (10−5 mol/L), coincubation of DHEA (10−5 mol/L) with insulin 100 ng/mL, and DHEA (10−5 mol/L) with insulin 10 ng/mL. INS = insulin. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions


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