1. Response of attrappc11/rog2 Mutants to Salt Stress.
Response of attrappc11/rog2 Mutants to Salt Stress. (A) and (B) Representative 8-d-old seedlings of the mutants attrappc11/rog2-2, attrappc11/rog2-4, attrappc11/rog2-5, and attrappc11/rog2-7 as well as the Col-0 wild-type (WT) and the complemented attrappc11/rog2-2 mutant (YFP-AtTRAPPC11/ROG2attrappc11/rog2-2) are shown (A). Eight days after germination (DAG), all four AtTRAPPC11/ROG2 mutants display reduced root growth, compared with WT and the complemented attrappc11/rog2-2 mutant (B). P < (***), one-sample Student’s t test, n = minimum 24 seedlings per genotype. Error bars represent se. (C) and (D) Root elongation shows hypersensitivity to salt treatment in attrappc11/rog2-2, attrappc11/rog2-4, attrappc11/rog2-5, and attrappc11/rog2-7 compared with the Col-0 wild type (WT), 3 and 5 days after transfer (DAT) of 3-d-old seedlings to plates containing 100 mM NaCl. The hypersensitivity of attrappc11/rog2-2 mutants was alleviated by complementation with YFP-AtTRAPPC11/ROG2. The arrowhead in (C) denotes the position of root tips upon transfer. P < (***), one-sample Student’s t test, n = minimum 24 seedlings per genotype. Error bars represent se. (E) Time lapse of attrappc11/rog2-2 root growth under 75mm NaCl treatment. Root elongation measurements over the course of 110 h (only diurnal time points) show hypersensitivity of attrappc11/rog2-2 mutants to inhibition of root elongation by 75mm NaCl treatment, compared with the Col-0 wild type (WT; see Supplemental Movie 4). n = 25 seedlings per genotype. Error bars represent sd. (F) The salt hypersensitivity of attrappc11/rog2-2 mutants is dose dependent with 100 mM NaCl treatments resulting in an inhibitory effect on root growth stronger than the inhibition caused by 50 mM NaCl, measured 5 d after treatment. P < (***), one-sample Student’s t test, n = 25 seedlings per genotype. Error bars represent se.
Michel Ruiz Rosquete et al. Plant Cell 2019;31:
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