1. An improved method for endothelial cell seeding on polytetrafluoroethylene small caliber vascular grafts 
Aviva Schneider, MSc, Raphael N. Melmed, MD, Herzl Schwalb, PhD, Matthias Karck, MD, Israel Vlodavsky, PhD, Gideon Uretzky, MD 
Journal of Vascular Surgery 
Volume 15, Issue 4, Pages (April 1992)
DOI: / (92)
Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

2. Fig. 1
Rotation device for lining small caliber cylinders of Gore-Tex (PTFE) prosthesis with ECM and endothelium. The device consists of four axes rotating at a velocity of four rotations/hour, to guarantee even distribution of adhering ECs. PTFE cylinders (6 cm length × 0.6 cm diameter) were used for lining experiments. Bovine corneal cells in DMEM containing 5% FCS, 10% CS, 4% dextran, and 10 ng/ml FGF were seeded at a concentration of 5 × 105 cells/cm2 on segments first coated with fibronectin.
Journal of Vascular Surgery  , DOI: ( / (92) )
Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

3. Fig. 2
Proliferation of bovine corneal ECs seeded on tissue culture plastic or PTFE. Bovine ECs were seeded (2.5 × 104 cells/well) on tissue culture wells or PTFE segments (precoated with various substrates). Seven days after seeding, the cells were dissociated with STV and counted. A, Nontreated; B, Fibronectin; C, Collagen; D, Fibronectin + collagen.
Journal of Vascular Surgery  , DOI: ( / (92) )
Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

4. Fig. 3
Attachment and proliferation of bovine aortic ECs on Gore-Tex (PTFE) disks coated with different biosubstrates. A, Adult bovine ECs were seeded (2 × 103 cells/well) on untreated PTFE, PTFE coated with ECM, or PTFE that was first coated with fibronectin and then by ECM. After 7 days in culture cells were dissociated with STV and counted. B, Adult bovine ECs seeded (2 × 103 cells/well) on untreated PTFE disks (control) or on PTFE disks coated with fibronectin, fibronectin + ECM, or Matrigel. After 1 day (“attachment”) and 8 days (“proliferation”) in culture, cells were dissociated with STV and counted. No FGF was added during this experiment.
Journal of Vascular Surgery  , DOI: ( / (92) )
Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

5. Fig. 4
Scanning electron micrographs of ECM formed on plain PTFE and on PTFE that was first coated with fibronectin. a, ECM secreted on PTFE previously coated with fibronectin. ECM covers the graft in an almost complete and continuous manner. Texture is thick and gaps (holes) in the matrix are few and small. (Original magnification × 400.) b, same as a, but original magnification × c, ECM secreted directly on plain PTFE. Texture is thinner and many gaps exist of a bigger size than those seen on ECM covering fibronectin-coated PTFE. (Original magnification × 300.) d, same as c, but original magnification × 2000.
Journal of Vascular Surgery  , DOI: ( / (92) )
Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

6. Fig. 5
Scanning electron micrographs of corneal and vascular ECs cultured on coated PTFE. A, Bovine corneal ECs seeded on PTFE tubes coated with fibronectin and grown (as described in “Material and Methods”). Seven days later tubes were opened lengthwise and fixed for electron microscopy. (Original magnification × 1000.) B, Bovine aortic ECs seeded on ECM produced by corneal ECs described in A. (Original magnification × 1000.)
Journal of Vascular Surgery  , DOI: ( / (92) )
Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions