1. Expression and functional characterization of Cu transporters CtrA2 and CtrC in A. nidulans.
Expression and functional characterization of Cu transporters CtrA2 and CtrC in A. nidulans. (A) and (B) RT-qPCR analysis was performed after growth of cultures in liquid MMPDR media (1 μM Cu) for 18 h at 37°C, followed by a 1-h induction performed with 100 μM BCS. (C) RT-qPCR analysis was performed after growth of cultures in liquid MMPDR media (0 μM Cu) for 24 h at 37°C. For panels A, B, and C, the tubA gene was used as an internal control. ***, P < 0.001 (Student's t test); error bars represent standard deviations of results from three independent replicates. (D) Colony morphologies of ΔAnmac1 mutant strains overexpressing ctrA2 and ctrC mutant strains grown on MMPDR media supplemented with various concentrations of Cu at 37°C for 40 h. (E) Effect of exogenous Cu supplementation on the colony morphologies of the ΔAnctrA2, ΔAnctrC, and ΔAnctrA2 ΔAnctrC mutants grown on MMPDR media at 37°C for 40 h.
Zhendong Cai et al. mSphere 2019; doi: /mSphere