1. RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis in ovarian cancer cells.
RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis in ovarian cancer cells. A, cell-cycle distribution of ES-2 ovarian cancer cells, either mock treated or treated with the indicated concentration of RA-9 for 18 hours, was evaluated by PI staining and flow cytometric analysis. Percentage of cells in G1 phase (M1), S phase (M2), and G2–M phase (M3). B, ES-2 ovarian cancer cells either mock treated or treated with the indicated concentrations of RA-9 for 18 hours. Harvested cells were stained with a FITC-conjugated antiactive caspase-3 monoclonal antibody and analyzed by flow cytometry. C, cell lysate from ES-2 ovarian cancer cells exposed to 5 μmol/L RA-9 over 24 hours was Western blotted with an antibody recognizing both the full-length and cleaved forms of PARP. Equivalent protein loading was verified by using an antibody directed against β-actin. D, quantification of the PARP/cl.PARP ratio in mock versus ES-2–treated cells.
Kathleen Coughlin et al. Clin Cancer Res 2014;20:
©2014 by American Association for Cancer Research