1. In the presence of human monocytes, estradiol increased breast cancer cell dissemination to the periphery via CCL2 and CCL5.
In the presence of human monocytes, estradiol increased breast cancer cell dissemination to the periphery via CCL2 and CCL5. A, zebrafish embryos were injected with MCF-7 cells with or without human monocytes and with or without estradiol (E2), as described in the Materials and Methods section, and the number of disseminated cells was counted (n = 12–31 in each group). Graphed data are presented as mean ± SEM. B, zebrafish embryos were injected with MCF-7 cells and human monocytes and treated with estradiol (E2), as described in the Materials and Methods section. Anti-CCL2, anti-CCL5, or isotype control IgG antibody (ISO; 5 μg/mL) was also added to the mixture injected into the zebrafish embryo. The number of disseminated cells was counted (n = 5–18 in each group). Graphed data are presented as mean ± SEM.
Susanne Svensson et al. Clin Cancer Res 2015;21:
©2015 by American Association for Cancer Research