1. DNA Technology Notes

2. Journal 3
Compare/contrast replication, transcription and translation.

3. Define Genetic Engineering
Process where DNA is split into fragments and new DNA pieces are inserted
Restriction enzymes cut DNA at certain places
Carriers are then used to insert DNA splices
Now referred to as recombinant DNA or transgenic

4. Applications of recombinant DNA
Bacteria: used to reduce frost damage to crops, break down pollutants, produce hormones and antibiotics
Plants: resistant to herbicides, increase protein content in grains
Animals: cloning of endangered species, replacement of genes which cause disorders

5. Describe DNA fingerprinting
Samples of DNA is exposed to restriction enzyme which cuts it into pieces
DNA is loaded into a gel and run through an electrophoresis machine
Since DNA is cut at certain sequences, each piece is a different length and weight
Pieces that are heavier stay at the top of the gel, lighter will “run” to the bottom
Every person is genetically different, so DNA fingerprints are different

6. DNA fingerprinting applications
Can be used to identify or eliminate suspects when genetic material has been collected at a crime scene
Can be used to determine paternity of a child, or establish other family relationships
Can be used to identify a deceased person

7. Describe karyotyping
Picture of chromosomes are taken from a cell sample, cut out and matched up in pairs
Humans have 23 pairs of chromosomes
Karyotypes can be used to determine if genetic disorder is present
If too many are present can indicate Down’s syndrome
If some are missing can indicate Turner’s syndrome

8. Gene therapy
Insertion of normal gene into human cell to correct genetic disorders
Being used now to treat cystic fibrosis

9. Quiz 3
In genetic ? DNA is split and new fragments of DNA are inserted.
a. deletion b. engineering c. mutation
2. Gene ? Involves the insertion of normal genes in the place of genes which control genetic disorders.
a. cloning b. mutation c.therapy
DNA fingerprinting works because everyone’s DNA is different except for ?
a. fraternal twins b. identical twins
The structure of DNA was discovered by ?
a. Watson & Crick b. Wilson & Crockett
c. Schleiden & Schwann
DNA gels can be used for ?
a. determination of parents b. elimination of suspects
c. identification of body d. all of these **