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Diisocyanate antigen–enhanced production of monocyte chemoattractant protein-1, IL- 8, and tumor necrosis factor-α by peripheral mononuclear cells of workers.

Published byFarida Agusalim Modified over 5 years ago

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Presentation on theme: "Diisocyanate antigen–enhanced production of monocyte chemoattractant protein-1, IL- 8, and tumor necrosis factor-α by peripheral mononuclear cells of workers."— Presentation transcript:

1 Diisocyanate antigen–enhanced production of monocyte chemoattractant protein-1, IL- 8, and tumor necrosis factor-α by peripheral mononuclear cells of workers with occupational asthma  Zana L. Lummus, PhDa, Rafeul Alam, MD, PhDb, Jonathan A. Bernstein, MDa, David I. Bernstein, MDa  Journal of Allergy and Clinical Immunology  Volume 102, Issue 2, Pages (August 1998) DOI: /S (98) Copyright © 1998 Mosby, Inc. Terms and Conditions

2 Fig. 1 Enhancement of MCP-1, MCP-3, MIP-1α, and RANTES secretion from PBMCs by in vitro culture with diisocyanate-HSA conjugated antigen for 18 hours. Enhancement is derived by subtraction of spontaneous production in media alone from diisocyanate-HSA–stimulated production. Mean values for 8 patients with DOA are compared with 4 asymptomatic diisocyanate-exposed workers and with 4 nonexposed normal subjects by Mann-Whitney test. Significant differences are indicated by asterisks (P < .05). Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

3 Fig. 2 Kinetics of synthesis of MCP-1 by PBMC cultures from 8 subjects with DOA. PBMC supernatants were tested after in vitro cell culture at 4 and 18 hours in medium alone (Medium), with PHA, with HSA, with diisocyanate-HSA conjugated antigen (DIISO), or with an irrelevant antigen conjugate consisting of phthallic anhydride conjugated to HSA (PA-HSA). Values are mean levels of total MCP-1 found in cell supernatants. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

4 Fig. 3 Ethidium bromide–stained PCR products after RT-PCR analysis for β-actin, MCP-1, RANTES (upper gels), MCP-3, and MIP-1α (lower gels) mRNA expression in PBMCs from asymptomatic control subject number 12 (lanes 1 and 2) and patient number 10 with DOA (lanes 3 and 4). Chemokine-positive control subjects are represented in lane 5. PBMCs were tested before culture (lanes 1 and 3) and after 18 hours of culture in medium containing HDI-HSA antigen (lanes 2 and 4). Total cellular RNA was reverse-transcribed, and cDNA was amplified by PCR with chemokine-specific primers. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

5 Fig. 4 Enhancement of IL-4, IL-5, IFN-γ, and TNF-α secretion from PBMCs by in vitro culture with diisocyanate-HSA–conjugated antigen for 18 hours. Enhancement is derived by subtraction of spontaneous production in media alone from diisocyanate-HSA–stimulated production. Mean values for 8 patients with diisocyanate-induced asthma are compared with 4 diisocyanate-exposed asymptomatic workers by the Mann-Whitney test. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

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