1. Rice OsGL1-1 Is Involved in Leaf Cuticular Wax and Cuticle Membrane
Qin Bao-Xiang , Tang Ding , Huang Jian , Li Ming , Wu Xin-Ru , Lu Li-Li , Wang Ke-Jian , Yu Heng-Xiu , Chen Jian-Min , Gu Ming-Hong , Cheng Zhu-Kuan  
Molecular Plant 
Volume 4, Issue 6, Pages (November 2011)
DOI: /mp/ssr028
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

2. Figure 1 Phenotype of the osgl1-1 Mutant.
(A) Phenotypic comparison between WT (left) and osgl1-1 (right).
(B) Comparison of wetting characteristics of leaf surfaces by water between WT (left) and osgl1-1 (right).
(C) Transgenic plant generated by complementation.
(D) Wetting characteristics of leaf surfaces of transgenic plant generated by complementation by water.Bars: (B, D), 5 mm.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
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3. Figure 2 Fine Mapping and Positional Cloning of the OsGL1-1 Gene.
(A) The OsGL1-1 locus was mapped on the chromosome 9 between markers P1 and P6.
(B) Fine mapping of OsGL1-1 was positioned on BAC3 (BAC OSJNBb0057D06) within a 35-kb region flanked by the markers P7 and P9.
(C) Only one candidate gene existed within the 35-kb restricted region.
(D) Mutation position in the OsGL1-1 coding region that is separated by nine introns.
(E) Complementation constructs. The construct pCGL contains the entire OsGL1-1 gene, plus a bp upstream region and bp downstream region.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
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4. Figure 3
Multiple Alignments of Plant Proteins Showing High Similarity with the OsGL1-1 Protein.
OsGL1-1 (top line) was aligned with three rice proteins (OsGL1-2, LOC_Os06g44300, and WDA1), two Arabidopsis proteins (CER1 and WAX2), and one maize protein (GL1). Identical residues are boxed in black and similar residues in gray. The conserved desaturase/hydroxylase domain is marked above the OsGL1-1 sequence with a gray line. Black segments identify conserved His residues within the desaturase/hydroxylase domain. Putative transmembrane domains (TM1–TM5) of OsGL1-1 are marked above the OsGL1-1 sequence with a darker gray line. The identity and similarity of the aligned proteins for OsGL1-1 are shown at the end of the alignments.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
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5. Figure 4 Phylogenic Analysis of Proteins Similar to OsGL1-1.
The deduced amino acid sequence of the OsGL1-1 was aligned against 13 deduced sequences obtained from homologous loci, as available in GenBank. Neighbor-joining analysis was used to obtain a phylogenetic tree, which was bootstrapped over cycles. Significance values above a 50% cutoff threshold are indicated near the relative branches.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
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6. Figure 5
RT–PCR Analysis of OsGL1-1 in Seedlings, Root, Culm, Leaf, and Panicle of WT, the Leaf of WT, and OsGL1-1.
UBQ gene was used as a control.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

7. Figure 6
Histochemical Staining of GUS Activity in POsGL1-1:GUS Transgenic Rice Plants.
GUS activity was detected in aerial parts and roots of seedlings (A), glumes (B), stigma, anthers, filament, ovary (C), young seed (D), older seed (E), internode of older stem (F), transversal section of the leaf (G), transversal section of older stem (H), and root (I). Bars: (A, B), 2 mm; (C), 0.5 mm; (D–F), 1 mm; (G, I), 10 μm; (H), 80 μm.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
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8. Figure 7 Electron Microscopic Analysis of WT and osgl1-1.
(A) Adaxial surface of WT.
(B) Abaxial surface of WT.
(C) TEM analysis of WT cuticle membranes.
(D) Adaxial surface of osgl1-1 mutant.
(E) Abaxial surface of osgl1-1 mutant.
(F) TEM analysis of osgl1-1 mutant cuticle membranes.
P,papillae; CW, cell wall; TL, translucent layer; OL, opaque layer. Bars: (A, B, D, E), 2.5 μm; (C, F), 200 nm.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
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9. Figure 8 Altered Cuticular Permeability in the osgl1-1 Mutant.
(A) Chlorophyll leaching assays with matured leaves of WT and osgl1-1 mutant, immersed in 80% ethanol for different time intervals. Data are shown by mean ± SE with three replicates.
(B) Water-loss rate of detached leaves of WT and osgl1-1 mutant; x-axis is the scale for different time points and the y-axis is percentage of free water loss from leaves. Data are shown by mean ± SE with three replicates.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

10. Figure 9
Effects of Drought Stress Comparison between WT and osgl1-1 Mutant.
(A) Six-week-old WT (left) and osgl1-1 mutant (right) before drought stress.
(B) WT (left) and osgl1-1 mutant (right) after re-watering for 14 d.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
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11. Figure 10
Sub-Cellular Localization of OsGL1-1–GFP in Onion Skin Epidermal Cells.
An onion skin epidermal cell expressing GFP alone (A), its DIC image (B), and merge (C), showing fluorescent signals in nucleus, membrane, and cytoplasm. An onion skin epidermal cell expressing OsGL1-1–GFP alone (D), its DIC image (E), and merge (F), showing a similar signal pattern.
Molecular Plant 2011 4, DOI: ( /mp/ssr028)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions