1. Influence of minocycline on TGF-β1 expression and TGF-β1–induced NF-кB activation in ovarian cancer cells.
Influence of minocycline on TGF-β1 expression and TGF-β1–induced NF-кB activation in ovarian cancer cells. A, confocal immunocytochemistry of TGF-β1 (green) in OVCAR-3 and SKOV-3 cells treated with minocycline in comparison with control cells. The nuclei are counterstained with propidium iodide (PI; red). Images were obtained at × 60 magnification. The scale bars represent 100 μm. B, the expression levels of TGF-β1 in cells treated with minocycline (0–100 μmol/L) as estimated by Western blot analysis. The densitometric values quantified using the Quantity One image program (Bio-Rad) were corrected on the basis of β-actin and were expressed as the percentage of the values corresponding to control cells. Columns, means of 3 independent experiments; bars, SD. **, P < 0.01 versus control cells. C, TGF-β1 expression in cells either untransfected or stably transfected with pCMV6-TGF-β/pCMV6-Neo as assessed by Western blotting. β-actin, loading control. D, NF-кB–dependent luciferase activity of TGF-β1–dominant cells treated with vehicle/minocycline (100 μmol/L) expressed as fold change comparing pCMV6-Neo–transfected cells. Results were standardized relative to protein concentration. Columns, means of 3 independent experiments; bars, SD. **, P < 0.01 and ***, P < versus pCMV6-Neo–transfected cells. E, TGF-β1 expression in cells either untransfected or stably transfected with Scrambled shRNA/TGF-β1-shRNA as assessed by Western blotting. β-actin, loading control. F, NF-кB activity of Scrambled shRNA/TGF-β1-shRNA–transfected cells treated with vehicle/minocycline (100 μmol/L) assessed by luciferase reporter assay. Results were standardized relative to protein concentration and expressed as fold change comparing with vehicle-treated cells in each group. Columns, means of 3 independent experiments; bars, SD. **, P < 0.01 and ***, P < versus vehicle-treated cells in each group.
Parvin Ataie-Kachoie et al. Mol Cancer Res 2013;11:
©2013 by American Association for Cancer Research