1. The phagocytes: neutrophils and monocytes
by David C. Dale, Laurence Boxer, and W. Conrad Liles
Blood
Volume 112(4):
August 15, 2008
©2008 by American Society of Hematology

2. David C. Dale. David C. Dale
David C. Dale et al. Blood 2008;112:
©2008 by American Society of Hematology

3. W. W. Laurence Boxer David C. Dale et al. Blood 2008;112:935-945
©2008 by American Society of Hematology

4. W. W. Conrad Liles David C. Dale et al. Blood 2008;112:935-945
©2008 by American Society of Hematology

5. Model for the total blood granulocyte pool (TBCG) in normal subjects.
Model for the total blood granulocyte pool (TBCG) in normal subjects. CGP indicates circulating granulocyte pool; MGP, marginal granulocyte pool; and BM, bone marrow. Illustration by Kenneth Probst. This research was originally published in Blood. Cartwright GE, Athens JW, Wintrobe MM. The kinetics of granulopoiesis in normal man. Blood. 1964;24: ©American Society of Hematology.16
David C. Dale et al. Blood 2008;112:
©2008 by American Society of Hematology

6. Colony assay led to the identification of the colony-stimulating factors beginning in the late 1960s. “Feeder” layers, containing various types of white blood cells in a semisolid medium, were placed in a small laboratory dish (1).
Colony assay led to the identification of the colony-stimulating factors beginning in the late 1960s. “Feeder” layers, containing various types of white blood cells in a semisolid medium, were placed in a small laboratory dish (1). Bone marrow cells (including stem cells) were added to form a second layer (2). When the dish was incubated, colonies of white blood cells formed in the second layer (3). The colonies were counted and the cells identified (4). When the contents of the first layer were varied, different types of colonies formed, implying the existence of a range of colony-stimulating factors. Illustration by Kenneth Probst. Adapted with permission from original artist Patricia J. Wynne. Golde DW, Gasson JC. Hormones that stimulate the growth of blood cells. Sci Am. 1998;259:67.25
David C. Dale et al. Blood 2008;112:
©2008 by American Society of Hematology

7. The MPO-H2O2-chloride antimicrobial system (taken from Goebel and Dinauer50).
The MPO-H2O2-chloride antimicrobial system (taken from Goebel and Dinauer50). NADPH indicates reduced nicotinamide adenine dinucleotide phosphate; O2, superoxide anion; and HOCI, hypochlorous acid. Illustration by Kenneth Probst. Adapted with permission from Journal of Leukocyte Biology. Klebanoff SJ. Myeloperoxidase: a friend and foe. J Leukoc Biol. 2005;77:
David C. Dale et al. Blood 2008;112:
©2008 by American Society of Hematology

8. Model of NADPH oxidase assembly.
Model of NADPH oxidase assembly. Activation/phosphorylation (P)-induced conformational changes in p47phox release autoinhibitory interactions to unmask essential binding domains and exposure of PX domains that facilitate membrane targeting and binding of SH3-mediated and non–SH3-mediated binding events. Final interaction of the p67phox and Rac with flavocytochrome b induces conformational change, resulting in electron flow. Illustration by Kenneth Probst. Adapted with permission from Journal of Leukocyte Biology. Quinn MT, Gauss KA. Structure and regulation of the neutrophil respiratory burst oxidase: comparison with nonphagocyte oxidases. J Leukoc Biol. 2004;76:
David C. Dale et al. Blood 2008;112:
©2008 by American Society of Hematology

9. Overview of the pattern-recognition receptor system of phagocytes, including the TLR family.
Overview of the pattern-recognition receptor system of phagocytes, including the TLR family. Illustration by Kenneth Probst. Adapted with permission from: Trinchieri G, Sher A. Cooperation of Toll-like receptor signals in innate immune defense. Nat Rev Immunol. 2007;7:
David C. Dale et al. Blood 2008;112:
©2008 by American Society of Hematology