1. Growth with N-acetylglucosamine rapidly raises the environmental pH and releases detectable ammonia.
Growth with N-acetylglucosamine rapidly raises the environmental pH and releases detectable ammonia. (A) Wild-type C. albicans strain SC5314 was assessed for pH neutralization in YNBAG plus 20 mM N-acetylglucosamine or 1% Casamino Acids over 24 h. pH was adjusted to 4 with HCl prior to inoculation. (B) Ammonia released by C. albicans cells during pH neutralization on solid medium containing YNBAG plus 20 mM N-acetylglucosamine or 1% Casamino Acids. Ammonia was detected after 24, 48, and 72 h via Nessler’s reagent. *, P < Data are expressed as mean values ± standard deviation (SD) from triplicate experiments. YNBAG medium consists of minimal YNB supplemented with 0.5% glycerol and 0.5% allantoin.
Elisa M. Vesely et al. mSphere 2017; doi: /mSphere