1. Supplementary Figure
Endoplasmic Reticulum Chaperone GRP78 Regulates Macrophage Function and Insulin Resistance in Diet-Induced Obesity
Jong Hun Kim, Eunjung Lee, Randall H. Friedline, Sujin Suk, Dae Young Jung,
Sezin Dagdeviren, Xiaodi Hu, Kunikazu Inashima, Hye Lim Noh,
Jung Yeon Kwon, Aya Nambu, Jun R. Huh, Myoung Sook Han, Roger J. Davis, Amy S. Lee, Ki Won Lee, and Jason K. Kim

2. Supplementary Figure 1 GRP78 Lyz2-Cre
Ladder f/+ f/+ f/f +/+
 GRP78 f (568 bp)
 GRP78 + (524 bp)
Lyz2-Cre
 Lyz2-Cre (500 bp)
Ladder ‒ ‒
Supplementary Figure 1. Generation of Lyz-GRP78-/- mice.
Genomic DNA isolated from the indicated mice was examined by PCR analysis.
Establishment of Lyz-Grp78 KO mice.
**P<0.01, ***P<0.001 vs. WT mice
WT (n=3-4), KO (n=3-4)

3. Supplementary Figure 2 HFD-Fed WT HFD-Fed Lyz-GRP78-/- GLUT4 GAPDHKO
Establishment of Lyz-Grp78 KO mice.
**P<0.01, ***P<0.001 vs. WT mice
WT (n=3-4), KO (n=3-4)
Supplementary Figure 2. Skeletal muscle GLUT4 expression in HFD-fed Lyz-GRP78-/- and WT mice.
Gastrocnemius tissue samples were collected at the end of hyperinsulinemic-euglycemic clamp, and
protein lysates were used to detect GLUT4 expression by Western blot analysis.