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Steroid receptor and aromatase expression in baboon endometriotic lesions  Asgerally T Fazleabas, Ph.D., Allison Brudney, B.S., Daniel Chai, D.V.M., David.

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Presentation on theme: "Steroid receptor and aromatase expression in baboon endometriotic lesions  Asgerally T Fazleabas, Ph.D., Allison Brudney, B.S., Daniel Chai, D.V.M., David."— Presentation transcript:

1 Steroid receptor and aromatase expression in baboon endometriotic lesions 
Asgerally T Fazleabas, Ph.D., Allison Brudney, B.S., Daniel Chai, D.V.M., David Langoi, D.V.M., Serdar E Bulun, M.D.  Fertility and Sterility  Volume 80, Pages (September 2003) DOI: /S (03)

2 FIGURE 1 Diagrammatic illustration of endometriosis induction and tissue collection in the baboon model. Fazleabas. Endometriosis in the baboon. Fertil Steril 2003. Fertility and Sterility  , DOI: ( /S (03) )

3 FIGURE 2 RT-PCR of steroid receptor and aromatase expression in matched samples of eutopic and ectopic endometrium. The reverse transcribed DNA was separated into five equal aliquots and amplified using the specific primers (Table 1). Lane 1 shows positive controls for each of the primers; ERα and PR (baboon endometrium from proliferative stage of the cycle), ERβ (proliferative stage ovary), aromatase (baboon placenta). Lane 2 represents a human endometriotic lesion, lane 3 is baboon menstrual endometrial tissue, lanes 4 and 6 are eutopic endometrium from baboons with endometriosis for 1 and 10 months, and lanes 5 and 7 are endometriotic lesions from the same animals. H3.3 is a histone gene used as an internal loading control for each of the tissues. Fazleabas. Endometriosis in the baboon. Fertil Steril 2003. Fertility and Sterility  , DOI: ( /S (03) )

4 FIGURE 3 Histology and steroid receptor immunocytochemistry. Gomori's trichrome-stained sections of baboon menstrual endometrium (A), an endometriotic lesion (B), and proliferative eutopic endometrium from a baboon with endometriosis (C). Additional sections from the same tissues were stained for ERβ (D, E, F), for ERα (G, H, I), and for PR (J, K, L). Magnification bar, 150 μm. gl = glands; st = stromal cells. Fazleabas. Endometriosis in the baboon. Fertil Steril 2003. Fertility and Sterility  , DOI: ( /S (03) )

5 FIGURE 4 Immunocytochemical localization of aromatase in the stromal cells of an endometriotic lesion (B) and in granulosa cells of a developing follicle (C, positive control). The histology of this lesion (A). st = stromal cells. Fazleabas. Endometriosis in the baboon. Fertil Steril 2003. Fertility and Sterility  , DOI: ( /S (03) )

6 FIGURE 5 Immunolocalization of glycodelin and α-SMA in eutopic endometrial sections from control animals and baboons with endometriosis. The endometrial tissues were obtained on day 10 PO after CG infusion. (A, C) Stained with a monoclonal antibody to glycodelin. Note the absence of glycodelin protein (arrows) in endometrial tissues of baboons with endometriosis (C). Also note the absence of α-SMA localization in the subepithelial stromal cells in the animals with endometriosis (D) compared to the CG-treated controls (B). sa = spiral artery; myo = myometrium. Fazleabas. Endometriosis in the baboon. Fertil Steril 2003. Fertility and Sterility  , DOI: ( /S (03) )


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