1. Fig. 7 BEL immune response.
BEL immune response. BAL was performed on BEL and native lungs of all animals. (A and B) Examination of IL-8, 1L-1β, IL-6, IL-10, IL-12p70, IL-2, IL-4, and interferon-γ (IFN-γ) concentrations in BAL of (A) native lungs and BELs before transplant. (B) Polar plot showing cytokine concentrations for native lungs and overlay of data from BALs evaluated at 10 hours, 2 weeks, 1 month, or 2 months after transplantation. The polar plot highlights each animal’s immune response. (A to D) BALs performed on native lung at the time of the pneumonectomy and on BEL after euthanasia. (C) Number of CD8+ cells isolated from BALs. (D) Percentage of CD4+, CD8+ T lymphocytes, perforin-positive cells, and CD20+ B lymphocytes are shown. (E and G) DAPI-stained controls and (F and H) representative images of CD8+ T lymphocytes (green) in tissues of (F) native lung or (H) BEL. (I) Averaged CD8+ lymphocyte counts for tissue sections from pigs 1, 2, 4, and 5 native lungs and BELs. Analyses to compare native lung to BEL per pig were done using Student’s t test.
Joan E. Nichols et al., Sci Transl Med 2018;10:eaao3926
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