1. NRG2 Affects Nitrate Accumulation and Uptake
NRG2 Affects Nitrate Accumulation and Uptake.(A) to (C) Nitrate content in seedlings (A), roots (B), and leaves (C).
NRG2 Affects Nitrate Accumulation and Uptake.(A) to (C) Nitrate content in seedlings (A), roots (B), and leaves (C). Wild-type and nrg2 mutant plants were grown on ammonium nitrate medium for 7 d and collected for nitrate concentration test. Error bars represent sd of biological replicates (n = 4). Asterisks indicate significant differences (P < 0.05) compared with the wild type (t test). FW, fresh weight.(D) and (E) Nitrate accumulation in wild type, nrg2, and chl1 mutants. Seedlings grown with 2.5 mM ammonium succinate for 7 d were treated with various concentrations of KNO3 for 2 h (D) or treated with 5 mM KNO3 for different times in the presence of ammonium succinate (E) and then collected for nitrate concentration test. The chl1-13 mutant was used as a control. Asterisks indicate significant differences (P < 0.05) between the wild type and two mutants.(F) and (G) Relative expression of NRT1.1(F) and NRT1.8(G). Wild-type and nrg2 mutant plants were grown on ammonium nitrate medium for 7 d, and then roots and shoots were collected separately for RNA extraction. The transcription levels of NRT1.1 and NRT1.8 were determined by qPCR. Error bars represent sd of biological replicates (n = 4). Asterisks indicate significant differences (P < 0.05) compared with the wild type (t test).
Na Xu et al. Plant Cell 2016;28:
©2016 by American Society of Plant Biologists