1. Supersensitive fluorescent semen analysis: validation on azoospermic and oligozoospermic samples 
Claire E. Beyer, B.Sc., Bettina Kayler, B.M.Sc., Elissa Osborne, Ph.D., Robert McLachlan, M.D., Tiki Osianlis, Ph.D. 
Fertility and Sterility 
Volume 98, Issue 4, Pages e1 (October 2012)
DOI: /j.fertnstert
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
(A) Under fluorescence, two small bright nuclei and one larger nucleus are visible. (B) Using phase-contrast microscopy, the two small bright nuclei have distinguishable sperm tails whereas the larger nucleus does not. A high volume of debris is observed using phase-contrast microscopy. This debris is not observed under fluorescence because not all debris absorbs the fluorescent Hoechst stain.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions