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Perturbations of natural killer cell regulatory functions in respiratory allergic diseases  Francesca Scordamaglia, MD, Mirna Balsamo, PhD, Antonio Scordamaglia,

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Presentation on theme: "Perturbations of natural killer cell regulatory functions in respiratory allergic diseases  Francesca Scordamaglia, MD, Mirna Balsamo, PhD, Antonio Scordamaglia,"— Presentation transcript:

1 Perturbations of natural killer cell regulatory functions in respiratory allergic diseases 
Francesca Scordamaglia, MD, Mirna Balsamo, PhD, Antonio Scordamaglia, MD, Alessandro Moretta, MD, Maria Cristina Mingari, PhD, Giorgio Walter Canonica, MD, Lorenzo Moretta, MD, Massimo Vitale, PhD  Journal of Allergy and Clinical Immunology  Volume 121, Issue 2, Pages (February 2008) DOI: /j.jaci Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Circulating CD56++CD16+/− NK cells are reduced in allergic patients. Percentages of CD56+CD3− NK cells in PBL (A) and of CD56++ cells within the NK cell population (B) are shown (healthy individuals, squares; patients, triangles). C, CD56 and CD16 expression in purified NK cells from a healthy representative individual (H7) and a representative individual with allergy (P10). The CD56++CD16+/− cell population is quantified. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Evaluation of NK-cell–mediated IFN-γ production in patients with allergy or healthy subjects. A, IFN-γ content in supernatants from allergic (black) or healthy (gray) NK cells cultured with DCs. B and C, NK cells stimulated by cytokines are analyzed by FACS for their IFN-γ content (patients, black; healthy, gray). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 NK cells of patients with allergy induce poor DC maturation. Cytofluorimetric analysis of CD86 expression on DCs that have been cultured either in the absence (DC) or in the presence of healthy (DC+hNK) or patient (DC+pNK) NK cells. On the left and the right sides are indicated the DC and the P/H pairs analyzed in each experiment. Numbers indicate the CD86 mean fluorescence intensity. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Analysis of the ability of patients' NK cells to kill iDCs. Patients (black) and healthy (gray) NK cells are compared in a cytolytic assay for their ability to kill allogeneic iDCs. NK cells from each P/H pair were assessed against a different iDC target. E/T ratio, 10/1. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Synoptic data of the NK cell phenotype and functions of allergic patients analyzed. Diamonds, Percent of CD56++ NK cells. Bars, Patient/healthy donor (P/H) values ratio related to all of the NK cell functions analyzed (data from Fig 2, B, are not reported because they are not statistically significant). Black, IFN-γ induced on NK-DC coculture. Striped, DC killing. Gray, CD86 mean fluorescence intensity (DC maturation). White, IL-12 produced by DCs on NK-cell stimulation. The solid line refers to data in the healthy controls (P/H ratio = 1). The dotted line indicates the mean percentage of CD56++ cells in healthy controls. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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