1. UAB Nanostring Laboratory
Directors: Eddy Yang, M.D., Ph.D.; Bruce Korf, M.D., Ph.D.
Contact: Eddy Yang-
Debbie Della Manna- phone:
Nanostring Technology
Results
This platform enables the profiling of hundreds of targets simultaneously with high sensitivity and precision, even in formalin fixed paraffin embedded (FFPE) tissues, without the need for amplification steps except in single cell or low cell number experiments. The system utilizes a novel digital technology that is based on direct multiplexed quantification of nucleic acids and provides highly reproducible data over 5 logs of dynamic range.
Fold change in gene expression
represented as heat maps
Significance of changes in 13 key cancer pathways with PanCancer Pathways
Design your own custom panel
or use a pre-made panel:
PanCancer:
Pathways: 770 genes, all 13 pathways: Notch, Wnt, HedgeHog, Chromatin Modification, Transcriptional Regulation, DNA Damage Control, TGF-b, MAPK, STAT, RAS, P13K, Cell Cycle, Apoptosis
Immune: Human or Mouse,770 genes: antigen processing, senscence, regulation; cell, macrophage, NK cell, B-cell, transporter, T-cell, microglial, leukocyte functions; pathogen defense, TNF superfamily, chemokines, cytokines, cytotoxicity, cell cycle, adhesion, TLR,
complement, interleukins
Progression : 770 genes total consisting of Angiogenesis (277 genes), ECM ( genes), EMT (269 genes) and Metastasis (173 genes)
Profiles: 180 genes, select 1 pathway: Adaptive Immunity, Innate Immunity, Cancer Metabolism, Intracellular signaling, Cellular Profiling, Wnt, DNA Damage and Repair + can add up to 30 more custom genes
Many Others including:
RNA:Protein Immune Cell Profiling: same 770 genes as PanCan Immune with 30 proteins simultaneously
miRGE: human, mouse, rat or fly; mRNA, 5-30 miRNA simultaneously
Human or Mouse Immunology (594/561 genes) Human Kinase (522 genes)
Human or Mouse Inflammation (249 /248 genes) Stem Cell (194 genes)
Human or Mouse or Rat miRNA (800/>600/423 genes) Gene Fusion
Human Cancer Reference (230 genes) Breast Cancer ER (196 genes)
Copy Number Variation (800 regions) lncRNA quantification (up to 800 )
Single Cell Gene (800 genes) ChIP-String assay (800 loci)
Key Advantages
only 100ng of total RNA is required
from cell lysates, fresh or frozen tissue, FFPE samples or whole blood lysates
No amplification done, more quantitative
Fewer replicates required