1. Distinct antibiotic pretreatments have differential impacts on C
Distinct antibiotic pretreatments have differential impacts on C. difficile colonization and cecal microbiota community structure.
Distinct antibiotic pretreatments have differential impacts on C. difficile colonization and cecal microbiota community structure. (A) CFU of C. difficile 630 in stool of infected mice following each antibiotic-pretreated group over 10 days of infection. Median and interquartile range are shown for each time point. Both cefoperazone and streptomycin pretreatments had more significantly detectable CFU on the final day than did clindamycin pretreatment (P < 0.001). (B) Relative abundance of family-level OTU taxonomic classification in each pretreatment group from 16S rRNA gene sequencing. (C) Quantification of terminal vegetative C. difficile CFU in cecal content across 18-h colonization models. Black lines indicate median values, and each pretreatment group had significantly greater detectable CFU than no-antibiotic controls. Significant differences in panel A were determined through permutational multivariate analysis of variance (PERMANOVA) with dynamic time warping, and those in panel C were found by Wilcoxon rank sum test with Benjamini-Hochberg correction when necessary. The limit of detection (LOD) was used in place of undetectable values for statistical testing.
Matthew L. Jenior et al. mSphere 2018; doi: /mSphere