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A novel rat model of abdominal aortic aneurysm using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure  Akiko.

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Presentation on theme: "A novel rat model of abdominal aortic aneurysm using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure  Akiko."— Presentation transcript:

1 A novel rat model of abdominal aortic aneurysm using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure  Akiko Tanaka, MD, Tomomi Hasegawa, MD, PhD, Zhi Chen, MD, Yutaka Okita, MD, PhD, Kenji Okada, MD, PhD  Journal of Vascular Surgery  Volume 50, Issue 6, Pages (December 2009) DOI: /j.jvs Copyright © 2009 Society for Vascular Surgery Terms and Conditions

2 Fig 1 A schematic drawing shows the rat abdominal aortic aneurysm model using a combination of intraluminal elastase-infusion and extraluminal calcium chloride exposure. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

3 Fig 2 Photographs show aneurysmal lesions of abdominal aorta at 4 weeks in each group. Group EC, Combined elastase and calcium chloride; group E, elastase; group C, calcium chloride. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

4 Fig 3 A, Histologic findings of abdominal aortic aneurysms (AAAs) are shown at 4 weeks after staining with hematoxylin and eosin (HE) for an assessment of aortic wall thickness, elastin von Gieson (EVG) staining for an assessment of elastin content, and picrosirius red (PSR) staining for an assessment of collagen content in group EC. Bar, 300 μm. B, Quantitative analysis is shown for the three variables. NS, Not significant. All data are expressed as mean ± standard error for 14 mice in group EC (elastin and calcium chloride combined), 12 in group E (elastin only), and 9 in group C (calcium chloride only). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

5 Fig 4 Protease activity and messenger RNA (mRNA) expression in abdominal aortic aneurysms at 1 week A, Intra-aortic mRNA expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and matrix metalloproteinase (MMP)-2 and MMP-9. B, Gelatin zymography documents gelatinolytic activities of MMP-2 and MMP-9. C, Densitometric analysis of the MMP activities. *P < .0001, **P < NS, Not significant. All data are expressed as mean ± standard error of the mean for 6 rats per group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

6 Fig 5 Inflammatory cell infiltration in abdominal aortic aneurysms at 1 week. A, Immunohistochemical staining of CD3 and CD11b. Bar, 100 μm. B, Quantitative analysis of CD3+ and CD11b+ cells. NS, Not significant. All data are expressed as mean ± standard error of the mean for 6 rats per group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

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