1. All Cells Are Coated with Glycans
Glycocalyx
Cytoplasm
Nucleus
Plasma Membrane
~50 nm
Endothelial Cell Glycocalyx
Audrey M. Glauert and G.M.W. Cook
Human lymphocyte
Broekhuizen et al (2009) Curr Opin in Lipidol 20:57
B. van den Berg

2. Symbol Nomenclature for Glycans

3. Mucopolysaccharidoses
Prevalence: 1:25,000
Lysosomal storage of glycosaminoglycans, organomegaly
Many clinical features, variation in clinical onset, severity and progression
Coarse facial features, short stature
Neurological complications, enlarged or diseased heart valves, recurring respiratory infections
Early mortality

4. Non-Reducing End BiomarkersNS 6S 2S
MPS VII
Sly
MPS IIIA
Sanfilippo
MPS IIIC
MPS II
Hunter
MPS I
Hurler
MPS IIID
MPS IIIB
-Glucuronidase
Sulfamidase
N-Acetyl
Transferase
6-sulfatase
-L-iduronidase
Uronate
2-O-sulfatase
-N-acetyl
glucosaminidase

5. Bootcamp Groups Groups (4) Samples (8) A Sun-Mi Taryn
1) Heart – Wildtype
2) Heart – MPS3a-/- B
So-young
Raquel
3) Brain – Wildtype
4) Brain – MPS3a-/- C
Philip
Patricia
5) Heart – Wildtype
6) Heart – MPS3a-/- D
Ryan
Henry
Joanna
7) Brain – Wildtype
8) Brain – MPS3a-/-