1. Lin28b promotes the positive selection of CD5+ ImmB cells in neonatal mice.
Lin28b promotes the positive selection of CD5+ ImmB cells in neonatal mice. (A) Representative histogram overlay of CD5 surface expression on 2-day-old NBM and 4-month-old ABM small pre-B (Lin−CD19+CD93+IgM−CD43−CD24highFSClow) and ImmB cells (ImmB) (Lin−CD19+CD93+IgM+CD24high). Lower panel shows the CD5 fluorescence intensity IQRs of the same populations. Q1, 25th percentile; Q3, 75th percentile; M, median; FMO, fluorescence minus one staining control. (B) CD5 levels on PtC liposome–reactive and total ImmB cells from 2-day-old WT NBM. (C) Histogram overlay of CD5 levels on 2-day-old NBM and ABM of the indicated genotypes. Lower panel shows the CD5 IQR. (D) Representative FACS plots showing the frequency of PerC CD5+ B-1 cells from 10-day-old mice. (E) Quantification of data shown in (D) (n = 4 to 7 from three experiments). (F) Quantification of relative forward scatter (FSC-A) of ImmB from mice of the indicated genotypes and ages. Data are shown as a value relative to the FSC-A of a representative Lin28b+/+ 2-day-old sample (n = 3 to 7 from three experiments). ns, not significant. **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ (G) Representative histogram overlays of CD5 surface expression on WT BM ImmB of the indicated ages.
Stijn Vanhee et al. Sci. Immunol. 2019;4:eaax4453
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