1. Amino acids are required for completion of SS differentiation and cell viability in very-low-glucose medium.
Amino acids are required for completion of SS differentiation and cell viability in very-low-glucose medium. SS parasites cultured in bloodstream form very-low-glucose medium, RPMIθ (which allowed manipulation of amino acid content) supplemented with or without glucose, proline (P, 4.6 mM), threonine (T, 3.4 mM), or proline and threonine for 2 days followed by transfer to amino acid-replete SDM79θ were scored for (A) outgrowth and (B) viability by flow cytometry and propidium iodide (PI) staining. (C) Assessment of parasite outgrowth and (D) viability after oxidative phosphorylation inhibition by treatment with oligomycin (either 100 ng/ml or 1 μg/ml) for 2 days in RPMIθ with proline and threonine (AA) followed by transfer to SDM79θ to provide amino acids for PF parasites. Bars indicate standard deviation in triplicate assays.
Yijian Qiu et al. mSphere 2018; doi: /mSphere