1. Fig. 5 Dcr-2 directly binds to Toll 3′UTR.
Dcr-2 directly binds to Toll 3′UTR. (A) SDS-PAGE analysis of purified His8–Dcr-2 and Coomassie brilliant blue staining. M, molecular mass marker; lane 1, His8–Dcr-2. (B) Gel mobility shift assay was performed to evaluate the capacity of His8–Dcr-2 to bind in vitro–transcribed Toll 3′UTR. Lane 1, Toll 3′UTR alone; lane 2, Toll 3′UTR in reaction buffer; lane 3, Toll 3′UTR in reaction buffer supplemented with His8–Dcr-2. Protein-bound and free RNA are indicated. (C) Schematic diagram of the plasmid that transcribes the mRNA containing the egfp ORF followed by the Toll 3′UTR. (D) The plasmid diagrammed in (C) was cotransfected with dcr-2 (lane 2) or ago2 (lane 3) dsRNA into cultured S2 cells. Cell lysates were prepared and subjected to Western blots using the indicated antibodies.
Zhaowei Wang et al. Sci Adv 2015;1:e
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