1. Investigation of reactivity of D14 HLA-A
Investigation of reactivity of D14 HLA-A*24:02 CTL clones against DMNT1-mutated and wild-type peptides.
Investigation of reactivity of D14 HLA-A*24:02 CTL clones against DMNT1-mutated and wild-type peptides. A, K562 cells transfected with HLA-A*24:02 cDNA and pulsed with DNMT1 wild-type and -mutated peptides (at 5 μM) were tested against D14 HLA-A*24:02–specific CTL clones (5,000/well). B, to assess the internal processing of the DMNT1-mutated and wild-type protein, TOPO-TA cloning of the mutated and wild-type DMNT1 cDNA sequences from D14 into expression vector pcDNA3.1/V5 HIS TOPO was carried out. Testing was performed against COS-7 cells transfected with HLA-A*24 or HLA-B*07, in order to show the specificity of the DMNT1 mutant clone.
Antonia L. Pritchard et al. Cancer Immunol Res 2015;3:
©2015 by American Association for Cancer Research