1. Regulation of antibacterial B cell responses in the peritoneal cavity via MyD88 signaling in FRCs.
Regulation of antibacterial B cell responses in the peritoneal cavity via MyD88 signaling in FRCs. Enumeration of IgM or IgG ASCs in the omentum (A) and peritoneal cavity (B) in mice lacking MYD88 expression in FRCs or in Cre-negative littermates (Ctrl) using ELISPOT analysis at the indicated days post-intraperitoneal (p.i.) immunization with S. Typhi OmpC/F. (C) Flow cytometry–based assessment of germinal center B cells in the indicated mouse strains after OmpC/F intraperitoneal immunization. Representative dot plot analysis on day 4 after immunization (left) and time course analysis of pooled data (right). (D and E) Serum titers of S. Typhi OmpC/F-specific IgM (D) and IgG (E) antibodies on day 4 after immunization. (F to H) C57BL/6 mice were treated with CCR2-specific depleting antibody (MC-21) or isotype control antibody (isotype), and B cell responses were analyzed at day 4 after immunization. (F) Flow cytometry–based assessment of germinal center B cells and enumeration of IgM or IgG ASC in the omentum (G) and peritoneal cavity (H). (I) ASC in the omentum of the indicated bone marrow chimeric mice on day 4 after immunization with S. Typhi OmpC/F. (J) Bacterial load in blood and liver of mice lacking MYD88 expression in FRCs or in Cre-negative littermates (Ctrl) on day 4 after intraperitoneal infection with 5 × 105 CFU of attenuated S. Typhimurium. Data are shown as mean values ± SEM and are pooled from 4 to 10 mice per group from two (D to H) or three (A to C, and I and J) independent experiments; statistical analysis was performed using Student’s t test (A to H, and J) or one-way ANOVA with Dunnett’s multiple comparison test (I) with *P < 0.05, **P < 0.01, and ***P <
Christian Perez-Shibayama et al. Sci. Immunol. 2018;3:eaar4539
Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works