1. H3K36me3 is not essential for LEDGF and MLL target gene occupancy.
H3K36me3 is not essential for LEDGF and MLL target gene occupancy. A, mouse MLL–AF9 leukemia cells were transduced with lentiviral vectors expressing the indicated shRNAs. Transcript levels for genes indicated below were quantified by qRT-PCR after 3 days of selection with puromycin, and expressed relative to control shRNA transduced cells. B, global levels of histone methylation were determined by Western blot analysis of MLL–AF9 leukemia cells transduced with SETD2 or control shRNAs. C, mouse HSPCs transformed by MLL–AF9 were transduced with lentiviral vectors expressing the indicated shRNAs. Colony numbers were quantified after 5 days of selection in methylcellulose culture with puromycin. Results are expressed relative to control shRNA transduced cells. D, MLL–AF9-transformed HSPCs transduced with SETD2 or control shRNAs were subjected to ChIP assays using the indicated antibodies. Promoter regions amplified by qPCR are indicated below the respective panels. MLLc, MLL carboxy protein.
Li Zhu et al. Cancer Discov 2016;6:
©2016 by American Association for Cancer Research