Presentation is loading. Please wait.

Presentation is loading. Please wait.

Analysis of dCas9 expression.

Published byJeffry Gilmore Modified over 5 years ago

Similar presentations

Presentation on theme: "Analysis of dCas9 expression."— Presentation transcript:

1 Analysis of dCas9 expression.
Analysis of dCas9 expression. (A) Growth analysis of wild-type L. plantarum WCFS1 (blue), IM133 (WCFS1 carrying pSIP-SH-dCas9 and pSgRNA-notarget; gray), IM132 (WCFS1 carrying only pSIP-SH-dCas9; orange), and IM167 (WCFS1 carrying pEV and pSgRNA-notarget; green). Levels of growth of noninduced cultures and induced cultures (25 ng/ml inducer pheromone SppIP; black outline) are shown. (B) Phase-contrast micrographs of wild-type L. plantarum WCFS1 cells and IM133. The scale bars represent 5 µm. Ine Storaker Myrbråten et al. mSphere 2019; doi: /mSphere

Download ppt "Analysis of dCas9 expression."

Similar presentations

Like.

Like.
PDF Has Found Its Receptor

PDF Has Found Its Receptor
ΔsimA yeast cells appear degraded in vivo.

ΔsimA yeast cells appear degraded in vivo.
Two parts of the proteomic tree with EVGs of putative Flavobacteriaceae phages. Two parts of the proteomic tree with EVGs of putative Flavobacteriaceae.

Two parts of the proteomic tree with EVGs of putative Flavobacteriaceae phages. Two parts of the proteomic tree with EVGs of putative Flavobacteriaceae.
Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ.

Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ.
Dibutyryl cAMP does not rescue staurosporine-induced filamentation of a cyr1Δ/cyr1Δ mutant. Dibutyryl cAMP does not rescue staurosporine-induced filamentation.

Dibutyryl cAMP does not rescue staurosporine-induced filamentation of a cyr1Δ/cyr1Δ mutant. Dibutyryl cAMP does not rescue staurosporine-induced filamentation.
Growth characteristics of strain 22A and its MDH mutant derivatives.

Growth characteristics of strain 22A and its MDH mutant derivatives.
Topisirovic et al., (2003) EMBO J,22:

Topisirovic et al., (2003) EMBO J,22:
PM analysis of wild-type E. coli K-12 and its rpoZ-defective mutant.

PM analysis of wild-type E. coli K-12 and its rpoZ-defective mutant.
5-ALA increased porphyrin production, which was further enhanced by vitamin B12 supplementation in acne-associated type IA-2 strains. 5-ALA increased porphyrin.

5-ALA increased porphyrin production, which was further enhanced by vitamin B12 supplementation in acne-associated type IA-2 strains. 5-ALA increased porphyrin.
Size profile of viral small RNAs obtained from control cells and RIG-I- and MDA5-deficient (KO) cells after infection for 24 h with SINV at an MOI of 0.1,

Size profile of viral small RNAs obtained from control cells and RIG-I- and MDA5-deficient (KO) cells after infection for 24 h with SINV at an MOI of 0.1,
Candidates with a PVM localization.

Candidates with a PVM localization.
Changes to the growth conditions break the circuit by changing host gene expression Changes to the growth conditions break the circuit by changing host.

Changes to the growth conditions break the circuit by changing host gene expression Changes to the growth conditions break the circuit by changing host.
Expression response of AT-rich genes to lanthanides.

Expression response of AT-rich genes to lanthanides.
SEM images of S. aureus ATCC 700699 cells left untreated (A) or treated with polymyxin B (PMB; 99.5 mg/liter) (B), FADDI-019 (1× MIC) (C), FADDI-019 (4×

SEM images of S. aureus ATCC cells left untreated (A) or treated with polymyxin B (PMB; 99.5 mg/liter) (B), FADDI-019 (1× MIC) (C), FADDI-019 (4×
Synthetic perturbation of DE and DV genes.

Synthetic perturbation of DE and DV genes.
Staurosporine-induced filamentation requires Cyr1 and PKA

Staurosporine-induced filamentation requires Cyr1 and PKA
The ΔsimA strain shows less cellular proliferation during macrophage infection and ruptured yeast cells. The ΔsimA strain shows less cellular proliferation.

The ΔsimA strain shows less cellular proliferation during macrophage infection and ruptured yeast cells. The ΔsimA strain shows less cellular proliferation.
IVA can localize to and encase the forespore in the absence of VM

IVA can localize to and encase the forespore in the absence of VM
Individual genes exhibit distinct stage-specific expression among female gametocytes. Individual genes exhibit distinct stage-specific expression among.

Individual genes exhibit distinct stage-specific expression among female gametocytes. Individual genes exhibit distinct stage-specific expression among.

Similar presentations

Ads by Google