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Volume 117, Issue 5, Pages 1098-1104 (November 1999)
Characterization of Helicobacter pylori PldA, a phospholipase with a role in colonization of the gastric mucosa Nick Dorrell, M.Celeste Martino, Richard A. Stabler, Stephen J. Ward, Zun W. Zhang, Andy A. McColm, Michael J.G. Farthing, Brendan W. Wren Gastroenterology Volume 117, Issue 5, Pages (November 1999) DOI: /S (99)70394-X Copyright © 1999 American Gastroenterological Association Terms and Conditions
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Fig. 1 Egg yolk agar clearing by H. pylori strains SS1 and ND3 (ureB). H. pylori strain ND5 (pldA) shows the loss of lecithinase activity. Some bacterial cells have been removed from the agar, showing the clearing of the egg yolk agar by SS1 and ND3, but not by ND5. Gastroenterology , DOI: ( /S (99)70394-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions
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Fig. 2 Quantitative determination of PLA2 activity in H. pylori lysates. The results are presented as units of PLA2 per milligram of the total protein content present in the lysates. Gastroenterology , DOI: ( /S (99)70394-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions
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Fig. 3 Quantitative determination of hemolytic activity of H. pylori strains, showing the level of hemolysis of red blood cells after 2 hours at 37°C when coincubated with either 2% (▨) or 4% (▩) H. pylori suspensions. The level of hemolysis is presented as a percentage of 100% hemolysis determined by sonication. Gastroenterology , DOI: ( /S (99)70394-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions
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Fig. 4 Serum anti–H. pylori whole cell immunoglobulin responses 8 weeks after infection. End-point antibody titers from individual mice are shown. Bars denote mean antibody responses. Gastroenterology , DOI: ( /S (99)70394-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions
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