1. dNSP16 mutant MERS-CoV infection produces minimal changes in early host responses.
dNSP16 mutant MERS-CoV infection produces minimal changes in early host responses. Changes in functional host gene clusters on the basis of RNA expression following infection at an MOI of 5 of Calu-3 2B4 cells with dNSP16 mutant MERS-CoV (left) or SARS-CoV (right) relative to the WT control virus. The heat map plots significant enrichment of clustered functional categories (as determined by David analysis) for each mutant over time. Only marginal changes were noted during the first 24 h for both SARS-CoV and MERS-CoV dNSP16 mutants. After 24 h (right), significant changes due to SARS-CoV were noted; MERS-CoV had a significant CPE after 24 h postinfection, precluding analysis.
Vineet D. Menachery et al. mSphere 2017; doi: /mSphere