1. Human melanoma induces paracrine β-catenin signaling activation in DCs in vitro.
Human melanoma induces paracrine β-catenin signaling activation in DCs in vitro. A, Wnt5a and FoxP3 gene expression analysis in human melanoma. Riker Dataset, Oncomine 4.4. P value, nonparametric Spearman correlation calculation. B, Wnt-β-catenin canonical signaling pathway (left). Hypothesis: Wnt5a-conditioned DCs promote Treg differentiation (right). C, human melanoma CM coincubated with TCF/LEF1-GFP–transfected COS7 reporter cell line and analyzed by flow cytometry (top) and β-catenin Western blot analysis (bottom). D, human melanoma CMs coincubated with a 293T-TCF/LEF1-luciferase reporter cell line. P, one-way ANOVA. E, Wnt5a ELISA of WM115 and WM266 CM (left). WM115 and WM266 CM coincubated with human monocyte-derived DCs and analyzed by β-catenin Western blot analysis (right). *, P < 0.05; ***, P <
Alisha Holtzhausen et al. Cancer Immunol Res 2015;3:
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