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COLONY MORPHOLOGY ON AGAR PLATE CULTURES
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Form of Colony
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Elevation of Colony
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Margin of Colony
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Surface of Colony
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There are three common shapes of bacteria 1-Coccus
having one of the following arrangements Diplococcus: a pair of cocci Streptococcus: a chain of cocci Tetrad: a square of 4 cocci Sarcina: a cube of 8 cocci Staphylococcus: cocci in irregular, often grape-like clusters
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Bacillus (rod) 2- Bacillus: a single bacillus Streptobacillus: bacilli in chains Coccobacillus: oval and similar to a coccus
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Spiral 3- Vibrio: an incomplete spiral or comma-shaped
Spirillum: a thick, rigid spiral Spirochete: a thin, flexible spiral
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Simple Stai Simple Staining and Bacterial Cell Morphology
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Preparing a smear for staining
Preparing a smear for staining. (The following procedure is used for all of our staining) 1. Flame (sterilize) your inoculating loop/needle before and after use.
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2. Prepare the smear If you have a solid culture (agar colony), place a small drop of water on a clean slide. Drag the sterile inoculating needle tip through the edge of colony. b. Gently spread the mixture into a circle. A loop of liquid culture can be placed directly on the slide and spread out.
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3. Let the smear air dry completely.
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4. Heat-Fix the smear. Smears are heat-fixed by quickly passing the slide through a flame two or three times. This causes the microbes to stick to the slide and not get washed off during the staining process.
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5. Stain the smear. Place the slide on a rack over the sink. Flood the smear with stain and let it for seconds. Rinse gently and blot dry.
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6. Observe the slide under low and high-dry lenses to locate, center, and focus the image.
Then, place a drop of oil directly on the stained smear .Turn the oil lens into position and fine focus to observe the cells.
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Coccus (cocci pl.)
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Bacillus (Bacilli pl.)
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Spirillum (Spirilli pl.)
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Name the bacterial morphologies (shapes and arrangements) seen here.
1 3 2 4 5 6 Answers: 1. Spirillum 2. Coccus 3. Bacillus 4. Diplobacillus 5. Streptobacillus 6. Diplococcus
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Fungi
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Aspergillus sp. Penicillium sp.
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