1. - In Clinic Titer Testing for Core Vaccines

2. - An Overview

3. Biogal Galed Labs. Experience -established in 1986.
•A leader in veterinary diagnostic kits.
•Serologic and direct evaluation of veterinary diseases.
•Customers > 50 countries.

4. Biogal’s Markets
> 50 distributors worldwide and 250 endusers directly

5. Biogal - Extensive Range of Unique Veterinary Diagnostic Solutions:
Molecular diagnostic, portable,
fast, in clinic/lab
Validate Before You Vaccinate
Portable, Compact, Fast
Semi Quantitative ELISA

6. Biogal – Kits Available:
Canine (ICH-CPV-CDV)
Feline (FPLV-FHV-FCV)
Canine Parvo & Distemper IgM
Canine Ehrlichia canis
Canine Leptospira
Feline T.gondi & C.psittaci
Feline Corona Virus (FIP)
Avian Chlamydophila psittaci
Canine Brucella
Leptospira
Ehrlichia canis
Leishmania infantum
Anaplasma platys
Babesia canis
Babesia gibsoni
Canine/Feline parvovirus
Mycoplasma haemofelis
FeLV DNA
soon – Distemper RNA

7. - About VacciCheck®

8. A Diagnostic Tool VacciCheck®: Semi Quantitative In Clinic Titer Test
Measures antibody levels in blood or serum
Based on unique ImmunoComb principle - solid-phase ELISA

9. Antibody Test Kit - Contents
Developing Plate
Comb
Tweezers
Comb
Scale
Pipette

10. Main Components
The developing plate contains the reagents needed for Comb development.
Antigens are attached to each tooth of the Comb. The placement of the antigens is shown by the labeling on the Comb.

11. The color intensity of spots corresponds to antibody level.
Analyzing Results
Example of a Developed Comb for testing
Feline Panleukopenia, Calici and Herpes Virus
The color intensity of spots corresponds to antibody level.
Control
Panleukopenia
Herpes
Calici

12. Scoring Results qualitative results Reading results by eye
semi-quantitative results
Reading results using CombScale
quantitative results
Reading results using CombScan

13. As appears in Vaccination Guidelines
In Clinic Titer Testing for Core Vaccines
As appears in Vaccination Guidelines

14. VacciCheck – Recommended by WSAVA
Source: WSAVA Vaccination Guidelines 2016
“Since publication of the 2010 guidelines there have been advances in the availability of rapid and simple in-practice serological test kits that can detect the presence of protective antibody specific for CDV, CAV and CPV-2 in individual dogs…….
Two commercially produced test kits are available and have been applied and validated in the practice and shelter setting (Gray et al. 2012, Litster et al. 2012) [EB1]. These test kits have proven popular with veterinarians who wish to be able to offer their clients an alternative to routine core revaccination at 3-yearly intervals,” 

15. Recommended by AAHA and WSAVA VACCINATION GUIDELINES GROUPS for:
• Determining whether a puppy/kitten has responded to vaccination.
• Testing of newly adopted adult dogs/cats of unknown vaccination history.
• VacciCheck INSTEAD of automatic triennial core revaccination for adult dogs.
• Management of outbreaks in shelters.

16. Puppy Titer Testing
Source: WSAVA Vaccination Guidelines 2016

17. Critical Q & A for Serology Core Vaccine Testing
Q&A: 85. In an animal that has completed its puppy/kitten shots, is a higher
antibody titre required to protect against heavy disease challenge?
For CDV, CAV-2, CPV-2 and FPV the answer is no. The presence of antibody (no
matter what the titre) indicates protective immunity and immunological memory
is present in that animal. Giving more frequent vaccines to animals in an attempt
to increase antibody titre is a pointless exercise. It is impossible to create ‘greater
immunity’ by attempting to increase an antibody titre.
Q&A: 86. Can we test dogs as an alternative to annual vaccination? We are concerned about the advice to only boost every 3 years.
Yes, certainly. There are now well-validated in-practice serological test kits that permit determination of the presence of protective serum antibody specific for CDV, CAV, CPV-2 and FPV. In other countries, these kits are used to confirm protection at 3-yearly intervals (instead of automatic revaccination for core diseases). You could perform serology annually, but if you were to collect and analyze the data that you generated within your practice, you will quickly find that annual testing is unjustified.
Source: Editorial WSAVA Vaccination Guidelines 2016

18. 2 of the 7 Indications for Serology Testing:

19. To assess the immunity
when there is a prior
vaccination history in an
adult dog but the dog is due
(or is overdue) for a
scheduled booster.

20. To assess the immunity following completion of the initial vaccination
series in puppies >24 wk of age

21. Key VacciCheck Clinical Papers
WSAVA recommendations for VacciCheck:
Read full article 
AAHA recommendations for VacciCheck:
Read full article
Professor Richard Ford Antibody Titers versus Vaccination Read full article
Professor Michael J. Day VacciCheck Used in a Major Clinical Study
Read

22. In the Press
One way to insure comfort for pet owners and veterinarians alike is to titer test dogs every three years. A titer test checks to determine the level of protective antibodies. If those antibodies exist, why revaccinate? Schultz asks. A new titer test, called Canine VacciCheck, just now being made available to veterinarians, is faster and easier, Schultz notes.