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Figure 3 Patient cells show impaired OXPHOS complex levels and function Patient cells show impaired OXPHOS complex levels and function (A) SDS-PAGE and.

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Presentation on theme: "Figure 3 Patient cells show impaired OXPHOS complex levels and function Patient cells show impaired OXPHOS complex levels and function (A) SDS-PAGE and."— Presentation transcript:

1 Figure 3 Patient cells show impaired OXPHOS complex levels and function
Patient cells show impaired OXPHOS complex levels and function (A) SDS-PAGE and immunoblotting were performed on 25 μg of isolated mitochondria from control and patient fibroblasts. (B) Mitochondrial protein synthesis was examined in control and patient fibroblasts using 35S radiolabeling of mitochondrial translation products. Twenty micrograms of cell lysate was separated on a 12.5% SDS-PAGE gel and visualized by autoradiography. (C) Oxygen consumption of specific respiratory complexes was measured in control and patient fibroblasts using an OROBOROS respirometer. (D) Phosphorylated (state 3) and nonphosphorylated (state 4) respiration was measured in the presence of 10 mM succinate/0.5 μM rotenone in digitonin-permeabilized control and patient cells to determine the respiratory control ratio under both glucose and galactose media conditions. Data are presented as mean ± SEM and were analyzed using a Student's t test. *p < 0.05, **p < 0.01. Richard G. Lee et al. Neurol Genet 2018;4:e276 Copyright © 2018 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.

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