1. Stable Cx43 expression in infected Cx43−/− cells and phosphorylation of Cx43.
Stable Cx43 expression in infected Cx43−/− cells and phosphorylation of Cx43. A. Immunoprecipitation was performed using affinity-purified rabbit anti-Cx43 antibody and proteins were resolved on a 12% SDS-polyacrylamide gel. Western blot was performed using rabbit polyclonal anti-Cx43 antibody. Cx43−/− pMSCV mock-infected cells (lane 1), Cx43−/− pMSCV Cx43 wild type (lane 2), and Cx43−/− pMSCV F199L Cx43 (lane 3). NP, non-phosphorylated form of Cx43; P1 and P2, slower migrating phosphorylated forms of Cx43. B. HeLa cells transiently transfected with Cx43 were metabolically labeled for 4 h. Anti-Cx43 immunoprecipitates were resolved on a 7.5 % polyacrylamide gel. Mock transfected (lane 1), Cx43 wild type (lane 2), and Cx43 F199L (lane 3). P0, P1, and P2, phosphorylated forms of Cx43.
Gordana Olbina, and Walter Eckhart Mol Cancer Res 2003;1:
©2003 by American Association for Cancer Research