1. Effect of two assisted oocyte activation protocols used to overcome fertilization failure on the activation potential and calcium releasing pattern 
Dimitra Nikiforaki, M.Sc., Ph.D., Frauke Vanden Meerschaut, M.D., Ph.D., Chloë de Roo, M.D., Yuechao Lu, M.D., Minerva Ferrer- Buitrago, M.Sc., Petra de Sutter, M.D., Ph.D., Björn Heindryckx, Ph.D. 
Fertility and Sterility 
Volume 105, Issue 3, Pages e2 (March 2016)
DOI: /j.fertnstert
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2. Figure 1
Calcium response in (A) mouse metaphase (M) II and (B) human MI-MII 3-hour oocytes after 10 μmol/L ionomycin or ready-to-use A23187 exposure. Each blue line indicates the Ca2+ response of an oocyte after the addition of 10 μmol/L ionomycin and each orange line after the addition of the ready-to-use A23187 solution.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
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3. Figure 2
Calcium response in human MI-MII 3-hours oocytes following the (A) ionomycin- or (B) ready-to-use A23187-based assisted oocyte activation (AOA) protocol. (A) The first Ca2+ rise is provoked by the intracytoplasmic sperm injection (ICSI) procedure combined with CaCl2 injection (black arrow). The second and third calcium rises are provoked by the first and second ionomycin exposures, respectively (blue arrows). The inset represents the first 1.5 hours of calcium measuring. (B) The first Ca2+ rise is provoked by the ICSI procedure (black arrow) and the second by the A23187 exposure (orange arrow). The inset represents the first 0.3 hours of calcium measuring. Abbreviations as in Figure 1.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

4. Supplemental Figure 1
The experimental approach using mouse and human oocytes that was followed in the present study. ICSI = intracytoplasmic sperm injection.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions