1. Effect of Mutations in Flavonoid Regulatory Genes on BAN Promoter Activity.(A) to (L) Pattern of GUS activity driven by the ProBAN1:uidA construct in regulatory mutant backgrounds.(A) tt2-1.(B) tt8-3.(C) ttg1-1.(D) ttg2-2.(E) to (I) tt16-1.(F) Arrowheads sh...
Effect of Mutations in Flavonoid Regulatory Genes on BAN Promoter Activity.(A) to (L) Pattern of GUS activity driven by the ProBAN1:uidA construct in regulatory mutant backgrounds.(A)tt2-1.(B)tt8-3.(C)ttg1-1.(D)ttg2-2.(E) to (I)tt16-1.(F) Arrowheads show elongated and vacuolated ii1 (black) and ii1′ (white) cells.(G) to (I) views of the same seed section showing daughter cells from a supernumerary periclinal division in a uidA-expressing cell (black arrowhead) and supernumerary layers in the inner integument (white arrowhead) (G), supernumerary layers in the inner integument (arrowhead) (H), and atypical localization of the first ii1′ cell in the inner integument (arrowhead) (I).(J) to (L)tt1-1.(J) Arrowheads show the absence of GUS activity in a group of endothelial cells.(K) Magnification of the chalazal region in (J).(L) Whole mount showing a lack of GUS activity at the base of the endothelium (arrowheads).(M) Pattern of GFP activity driven by the ProBAN1:mGFP5-ER construct in tt1-1, confirming the lack of ProBAN activity at the base of the endothelium (arrowheads).(N) to (O) Pattern of GUS activity driven by the ProTT1:uidA construct in the wild type.(N) Section of a seed at the globular stage.(O) Detailed view of the micropylar/chalazal area.(P) Whole mount of a seed at the globular stage observed with Nomarski optics.C, chalaza; CEC, chalazal endosperm cyst; CPT, chalazal proliferating tissue; EM, embryo; ii, inner integument; M, micropyle; N, nodule; oi, outer integument; PS, pigment strand; SC, supernumerary cell. Bar in (P) = 75 μm for (A) to (E), (J), (L) to (N), and (P), 35 μm for (F) to (I) and (O), and 20 μm for (K).
Isabelle Debeaujon et al. Plant Cell 2003;15:
©2003 by American Society of Plant Biologists