1. Organization of a typical IS
Organization of a typical IS. The IS is represented as an open box in which the terminal IRs are shown as grey boxes labelled IRL (left inverted repeat) and IRR (right inverted repeat).
Organization of a typical IS. The IS is represented as an open box in which the terminal IRs are shown as grey boxes labelled IRL (left inverted repeat) and IRR (right inverted repeat). A single open reading frame encoding the transposase is indicated as a hatched box stretching along the entire length of the IS and extending within the IRR sequence. XYZ enclosed in a pointed box flanking the IS represents short DR sequences generated in the target DNA as a consequence of insertion. The Tpase promoter, p, which is partially localized in IRL, is shown by a horizontal arrow. A typical domain structure (grey boxes) of the IRs is indicated beneath. Domain I represents the terminal base pairs at the very tip of the element whose recognition is required for Tpase-mediated cleavage. Domain II represents the base pairs necessary for sequence-specific recognition and binding by the Tpase.
Jacques Mahillon, and Michael Chandler Microbiol. Mol. Biol. Rev. 1998; doi: