Presentation is loading. Please wait.

Presentation is loading. Please wait.

Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Jarle Vaage, MD, PhD 

Published byTruls Kleppe Modified over 5 years ago

Similar presentations

Presentation on theme: "Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Jarle Vaage, MD, PhD "— Presentation transcript:

1 Induction of inflammatory mediators during reperfusion of the human heart 
Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Jarle Vaage, MD, PhD  The Annals of Thoracic Surgery  Volume 71, Issue 1, Pages (January 2001) DOI: /S (00)

2 Fig 1 Polyacrylamide gel with RT-PCR products of left ventricular biopsies of 1 patient with unstable angina scheduled for acute CABG. [33P]dATP was incorporated to detect PCR products in the linear phase of the PCR. In left ventricular biopsies before cardioplegia (B) and after reperfusion (A), primers for housekeeping gene histone H3 (215 bp), E-selectin (CD62E, 255 bp), eNOS (737 bp), IL-1β (464 bp), TNFα (444 bp), CD25 (312 bp), ICAM-1 (237 bp), CD18 (379 bp), iNOS (532 bp), and ET-1 (725 bp) were added. The ratios between H3 and test genes were calculated to evaluate changes in mRNA. The Annals of Thoracic Surgery  , DOI: ( /S (00) )

3 Fig 2 Changes in gene expression in left ventricular biopsies of 19 patients undergoing open heart surgery with cardioplegic arrest. The factor increase or decrease during reperfusion is calculated as ratio test gene/reference gene during reperfusion/ratio before cardioplegia. The effect of cardioplegia-reperfusion on E-selectin (CD62E), eNOS, IL-1β, TNFα, CD25, ICAM-1, CD18, iNOS, and ET-1 are shown (mean ± SEM). ∗p < 0.05 compared with initial expression. The Annals of Thoracic Surgery  , DOI: ( /S (00) )

4 Fig 3 Changes in gene expression in left ventricular biopsies of 7 patients with unstable angina (UA) and 7 patients with stable angina (SA) after cardioplegic arrest and reperfusion (see also text to Fig 2) (mean ± SEM). ∗p < 0.05 compared with initial expression. The Annals of Thoracic Surgery  , DOI: ( /S (00) )

5 Fig 4 Identification of NFκB complexes in nuclear extracts from human right and left atrial tissue sampled during open heart surgery. The extracts were resolved by EMSA as described in Material and Methods. Extracts from right atrial auricles taken before cardioplegia (B) and from left atrial auricles after reperfusion (A) are shown in 2 patients (nos. 1 and 2). Supershifts were performed with antibodies against the p50 and p65 subunits of NFκB on the A samples. Cold probe competition on the two A samples using a 25- (25cp) or 50-fold (50cp) excess of unlabeled probe abolished the bands, whereas they were not influenced by a 25- or 50-fold excess of an unlabeled AP-1 probe (25AP, 50AP). The Annals of Thoracic Surgery  , DOI: ( /S (00) )

6 Fig 5 The optical density of NFκB bands taken before cardioplegia (B) and after reperfusion (A) in 10 patients. Mean ± SEM values are shown. ∗p < The Annals of Thoracic Surgery  , DOI: ( /S (00) )

Download ppt "Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Jarle Vaage, MD, PhD "

Similar presentations

Different signalling pathways regulate VEGF and IL-8 expression in breast cancer: implications for therapy  Dina Chelouche-Lev, Claudia P. Miller, Carmen.

Different signalling pathways regulate VEGF and IL-8 expression in breast cancer: implications for therapy  Dina Chelouche-Lev, Claudia P. Miller, Carmen.
Patients with ulcerative colitis responding to steroid treatment up-regulate glucocorticoid receptor levels in colorectal mucosa  L. Flood, E. Innala,

Patients with ulcerative colitis responding to steroid treatment up-regulate glucocorticoid receptor levels in colorectal mucosa  L. Flood, E. Innala,
Microwave Ablation for Atrial Fibrillation: Dose-Response Curves in the Cardioplegia- Arrested and Beating Heart  Sydney L. Gaynor, MD, Gregory D. Byrd,

Microwave Ablation for Atrial Fibrillation: Dose-Response Curves in the Cardioplegia- Arrested and Beating Heart  Sydney L. Gaynor, MD, Gregory D. Byrd,
Effects of pleiotrophin, a heparin-binding growth factor, on human primary and immortalized chondrocytes  Dr. T. Pufe, Ph.D., G. Groth, M.B. Goldring,

Effects of pleiotrophin, a heparin-binding growth factor, on human primary and immortalized chondrocytes  Dr. T. Pufe, Ph.D., G. Groth, M.B. Goldring,
Induction by left ventricular overload and left ventricular failure of the human Jumonji gene (JARID2) encoding a protein that regulates transcription.

Induction by left ventricular overload and left ventricular failure of the human Jumonji gene (JARID2) encoding a protein that regulates transcription.
L. J. Sandell, Ph. D. , X. Xing, M. D. , C. Franz, M. A. , S

L. J. Sandell, Ph. D. , X. Xing, M. D. , C. Franz, M. A. , S
Volume 59, Issue 3, Pages (March 2001)

Volume 59, Issue 3, Pages (March 2001)
Modulation by aspirin of nuclear phospho–signal transducer and activator of transcription 6 expression: Possible role in therapeutic benefit associated.

Modulation by aspirin of nuclear phospho–signal transducer and activator of transcription 6 expression: Possible role in therapeutic benefit associated.
Epigenetic regulation of established human type 1 versus type 2 cytokine responses  Ruey-Chyi Su, PhD, Allan B. Becker, MD, Anita L. Kozyrskyj, PhD, Kent.

Epigenetic regulation of established human type 1 versus type 2 cytokine responses  Ruey-Chyi Su, PhD, Allan B. Becker, MD, Anita L. Kozyrskyj, PhD, Kent.
George D. Webb, PhD, Lay Har Lim, BSc, Vernon M. S

George D. Webb, PhD, Lay Har Lim, BSc, Vernon M. S
Acidosis-induced apoptosis in human and porcine heart

Acidosis-induced apoptosis in human and porcine heart
Celsior versus custodiol: early postischemic recovery after cardioplegia and ischemia at 5°C  Juergen Ackemann, MD, Wolfgang Gross, PhD, Martin Mory,

Celsior versus custodiol: early postischemic recovery after cardioplegia and ischemia at 5°C  Juergen Ackemann, MD, Wolfgang Gross, PhD, Martin Mory,
Volume 118, Issue 4, Pages (April 2000)

Volume 118, Issue 4, Pages (April 2000)
Signal transduction pathways triggered by the FcϵRIIb receptor (CD23) in human monocytes lead to nuclear factor-κB activation  Rosa M. Ten, MD, PhDa,

Signal transduction pathways triggered by the FcϵRIIb receptor (CD23) in human monocytes lead to nuclear factor-κB activation  Rosa M. Ten, MD, PhDa,
Constitutive and tumor necrosis factor-α-induced activation of nuclear factor-κB in adenomyosis and its inhibition by andrographolide  Bin Li, M.S., Ming.

Constitutive and tumor necrosis factor-α-induced activation of nuclear factor-κB in adenomyosis and its inhibition by andrographolide  Bin Li, M.S., Ming.
Kwo-Yih Yeh*,‡, Mary Yeh*, Jonathan Glass*, D.Neil Granger‡ 

Kwo-Yih Yeh*,‡, Mary Yeh*, Jonathan Glass*, D.Neil Granger‡ 
Revascularization of severe hibernating myocardium in the beating heart: early hemodynamic and metabolic features  Evasio Pasini, MD, Gianna Ferrari,

Revascularization of severe hibernating myocardium in the beating heart: early hemodynamic and metabolic features  Evasio Pasini, MD, Gianna Ferrari,
NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes  Dr C. Lianxu, Ph.D.,

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes  Dr C. Lianxu, Ph.D.,
Vein Graft Harvesting Induces Inflammation and Impairs Vessel Reactivity  Kazuhiro Hinokiyama, MD, Guro Valen, MD, PhD, Shinichi Tokuno, MD, PhD, Jenny.

Vein Graft Harvesting Induces Inflammation and Impairs Vessel Reactivity  Kazuhiro Hinokiyama, MD, Guro Valen, MD, PhD, Shinichi Tokuno, MD, PhD, Jenny.
Warm-blood cardioplegic arrest induces selective mitochondrial translocation of protein kinase Cε followed by interaction with 6.1 inwardly rectifying.

Warm-blood cardioplegic arrest induces selective mitochondrial translocation of protein kinase Cε followed by interaction with 6.1 inwardly rectifying.

Similar presentations

Ads by Google