1. Recent Developments in Single-Cell RNA-Seq of Microorganisms
Yi Zhang, Jiaxin Gao, Yanyi Huang, Jianbin Wang 
Biophysical Journal 
Volume 115, Issue 2, Pages (July 2018)
DOI: /j.bpj
Copyright © 2018 Biophysical Society Terms and Conditions

2. Figure 1
Workflow, single-cell sequencing platforms, and representative cDNA synthesis and amplification approaches for microorganisms. (A) A summarized pipeline showing the essential steps required to generate a cDNA library for next-generation sequencing is shown. (B) Two popular microfluidic-based sample processing platforms for single-cell sequencing are shown: Fluidigm IFC-C1 and Drop-seq. (C) The procedures used for global amplification of transcripts for RNA-seq of single eukaryote and prokaryote cells are shown (for further details, see text). They are as follows: (I) Rolling circle amplification (RCA); (II) RNA-based, single-primer, isothermal amplification (Ribo-SPIA); (III) Template-switching. To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2018 Biophysical Society Terms and Conditions