1. TNF-dependent communication between FALC FRCs and myeloid cells.
TNF-dependent communication between FALC FRCs and myeloid cells. C57BL/6 (B6) (A, B, and E to G) or Cc19-EYFP (C and D) mice were treated with the indicated fusion proteins 1 day before immunization with S. Typhi OmpC/F. (A and B) Accumulation of CD11b+F4/80−Ly6G−Ly6C+ inflammatory monocytes in the omentum; relative frequency (A) and absolute numbers (B) of inflammatory monocytes in the omentum. (C) Frequency of PDPN+EYFP+ cells in the omentum after TNFR-Ig treatment as determined by flow cytometric analysis. (D) Number of FALCs per square millimeter (left) and percentage of FALC-covered area in the omentum (right). Enumeration of IgM and IgG ASCs in the omentum (E) and the peritoneal cavity (F) and flow cytometry–based analysis of germinal center–like B cells (G) on day 4 after immunization in TNFR-Ig-treated mice. (H to K) Assessment of CD11b+ myeloid cell (H) and inflammatory monocyte recruitment (I) and accumulation of germinal center B cells (J) and IgG-producing B cells (K) in the omentum of the indicated bone marrow chimeric mice on day 4 after S. Typhi OmpC/F immunization. Data are shown as mean values ± SEM and are pooled from 7 to 8 mice from three independent experiments (B, C, and E to G), from 4 to 6 mice from two independent experiments (C and D), and from 7 to 12 mice from three independent experiments (H to K); statistical analysis was performed using Student’s t test (C to G) or one-way ANOVA with Dunnett’s multiple comparison test (A, B, and H to K) with *P < 0.05, **P < 0.01, and ***P <
Christian Perez-Shibayama et al. Sci. Immunol. 2018;3:eaar4539
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