1. related to Fig 1. Isolation and characterization of FAPs.
related to Fig 1. Isolation and characterization of FAPs. (A) Schematic representation of cell isolation strategy. (B) Flow cytometry analysis of FAPs plated in growth medium (DMEM + 20% FBS) for 4 d and stained with antibodies raised against CD140a and α7-integrin. (C) CD31−/CD45−/a7-integrin−/SCA1+ cells were cultivated in growth and adipogenic media for 8 d and stained with antibodies against the MYHC and with DAPI. Scale bar: 100 μm. (D) Differentiation potential of CD31−/CD45−/a7-integrin−/SCA1 cells. Cells were cultivated for 8 d to differentiate spontaneously in growth medium or were induced to differentiate into adipocytes (adipogenic medium) or into fibroblasts (growth medium supplemented with 10 ng/ml TGFb). Adipocytes were identified with ORO stain, and fibroblasts were stained with fluorescent antibodies against smooth muscle actin (SMA). Nuclei were counterstained with DAPI. Scale bar: 100 μm. (E) Percentage of adipocytes and SMA covered area are presented on graphs. Quantification of the immunofluorescence images are presented as average ± SEM of three biological replicas (n = 3). Statistical significance was evaluated by the ANOVA test (*P < 0.05).
Milica Marinkovic et al. LSA 2019;2:e
© 2019 Marinkovic et al.