1. Comparison of 16S sequencing and shallow shotgun recovery of species-level taxa.
Comparison of 16S sequencing and shallow shotgun recovery of species-level taxa. (A) Histogram of average Pearson correlation (R-squared) of species profiles between 16S sequencing and shallow shotgun sequencing from the same HMP sample (R-squared = 0.918), compared to the permutation-based null distribution of R-squared values for random pairings (P < 0.001). (B) Scatterplot of relative abundances of species in shallow shotgun sequencing versus 16S sequencing from the same HMP samples. Species found in only one data type are shown in a different color. (C) Fractions of all observed species, with relative abundance accounted for by species found by 16S sequencing only, shallow shotgun sequencing only, or both.
Benjamin Hillmann et al. mSystems 2018; doi: /mSystems