1. GBM 112 and GBM 144 cells infected in vitro with HCMV resist growth inhibition by temozolomide.
GBM 112 and GBM 144 cells infected in vitro with HCMV resist growth inhibition by temozolomide. (A) Sphere formation assays were conducted as described in the legend to Fig. 6 in the presence of carrier (DMSO [D]) or temozolomide (T) where indicated. The average percentages of sphere-forming cells from three biological replicates of GBM 112 and GBM 144 cells (mock infected or infected with HCMV TB40/E) are plotted with error bars representing the standard errors of the means. ***, P < 0.001; **, P < 0.01; NS, not significant by Student’s t test. (B) Sphere formation assays were conducted as described above with the addition of ganciclovir (G) where indicated. The average percentages of sphere-forming cells from three biological replicates from GBM 112 cells (mock infected or infected with HCMV TB40/E) are plotted with error bars representing the standard errors of the means. ***, P < 0.001; **, P < 0.01; *, P < 0.05; NS, not significant by Student’s t test.
Che Liu et al. mSphere 2017; doi: /mSphere