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Asthma is induced by intranasal coadministration of allergen and natural killer T-cell ligand in a mouse model  Jae-Ouk Kim, PhD, Dong-Hyeon Kim, MS,

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Presentation on theme: "Asthma is induced by intranasal coadministration of allergen and natural killer T-cell ligand in a mouse model  Jae-Ouk Kim, PhD, Dong-Hyeon Kim, MS,"— Presentation transcript:

1 Asthma is induced by intranasal coadministration of allergen and natural killer T-cell ligand in a mouse model  Jae-Ouk Kim, PhD, Dong-Hyeon Kim, MS, Woo-Sung Chang, BS, Changwan Hong, MS, Se-Ho Park, PhD, Sanghee Kim, PhD, Chang- Yuil Kang, PhD  Journal of Allergy and Clinical Immunology  Volume 114, Issue 6, Pages (December 2004) DOI: /j.jaci Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 Analysis of antigen+αGC-induced airway responses in BALB/c mice. A, AHR reactivity to nebulized methacholine. B, The number of total cell count (TCC), eosinophils (Eos), monocyte/macrophage (Mo), and lymphocytes (Lym) in BALF. C, Ovalbumin (OVA)-specific IgG and IgE levels in serum. In the IgE graph, the x-axis represents the dilution of serum. ▪, OVA+αGC; □, OVA; ▵, αGC; •, BSA+αGC; ×, Nil. D, Comparison of the OVA-specific cytokine production of BLN cells. E, Comparison of the OVA-specific proliferative ability of each group. n=3-5 mice in each group. Conc, Concentration. ∗P < .01 compared with all other groups. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Histologic examination of lungs. On day 21, lung tissue was removed from individual mice, formalin-fixed and paraffin-embedded, and periodic acid Schiff–stained (×200). Lung tissue from ovalbumin (OVA)+αGC (A), OVA (B), αGC (C), and Nil (D). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 Analysis of antigen+αGC-induced airway responses in CD1d-deficient mice. A, The airway reactivity to nebulized methacholine. B, The number of total cell count (TCC), eosinophils (Eos), monocytes/macrophages (Mo), and lymphocytes (Lym) in BALF on day 21. C, Ovalbumin-specific IgG and IgE levels in serum. In the IgE graph, the x-axis indicates the dilution of serum. ♦, CD1d+/−; ⋄, CD1d−/−. ∗P < .01; †P < .05 compared with the CD1d−/− group. Penh, Enhanced pause. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 Analysis of antigen+αGC-induced airway responses in MHC class II–deficient mice. A, The number of total cell count (TCC), eosinophils (Eos), monocytes/macrophages (Mo), and lymphocytes (Lym) in BALF. B, Ovalbumin-specific IgG and IgE levels in serum. In the IgE graph, the x-axis indicates the dilution of serum. n=6 per group. WT, Wild-type mice. ∗P < .01 compared with the MHC II−/− group. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

6 Fig 5 Changes in the NKT cell ratio in BLN and BALF of BALB/c mice. A, Comparison of BALF cells at indicated time points (day 0, before sensitization; day 3, 5, and 14, before inhalation of ovalbumin). B, The number of BLN cells at indicated time points. C, The increase of NKT cells in BALF. n=5-10 mice per group. ∗P < .01 compared with day 3; †P < .05 compared with day 14. TCC, Total cell count; Neu, neutrophils; Eos, eosinophils; Mo, monocytes/macrophages; Lym, lymphocytes. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

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