1. Selected metabolic features of the unFirm_1 phylotype as inferred from genome and proteome comparisons.
Selected metabolic features of the unFirm_1 phylotype as inferred from genome and proteome comparisons. The unFirm_1 phylotype encoded limited sugar utilization capabilities; however, a complete WL pathway and enzymes inferred to function in fatty acid degradation were observed. Metaproteomic analysis (genes, enzyme complexes, and transport systems are highlighted to indicate quantitative MaxQuant LFQ values) indicated that unFirm_1 was much more detectable in the Link_ADIb sample, with all WL pathway enzymes being detected, as well as genes associated with fatty acid degradation. In particular, the high relative abundance of a unidirectional fructose 1,6-bisphosphate aldolase/phosphatase (ALDOA) supported the hypotheses that unFirm_1 performs gluconeogenesis (anabolic glucose metabolism). Broken lines indicate annotations for which representative genes were not identified in the respective reconstructed genomes. Gene names and identification (IMG gene ID) numbers can be found in Table S1 in the supplemental material. THF, tetrahydrofolate; FTHFS, formyl-tetrahydrofolate synthase; PK, pyruvate kinase; PEP, phosphoenolpyruvate; G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; M6P, mannose-6-phosphate; PMM, phosphomannomutase; ACSL, long-chain-fatty-acid–CoA ligase; HADH, 3-hydroxyacyl-CoA dehydrogenase; GMPPB, mannose-1-phosphate guanylyltransferase; ACAT, acetyl-CoA acetyltransferase; XLF, d-xylulose; G3H, d-glyceraldehyde 3-phosphate; E4P, d-erythrose 4-phosphate; 5SP, d-xylulose 5-phosphate; S7P, sedoheptulose 7-phosphate; 5RP, d-ribulose 5-phosphate; RP5, d-ribose 5-phosphate.
J. A. Frank et al. mSystems 2016; doi: /mSystems